- pGLO - Wikipedia, the free encyclopedia
GFP can be induced in bacteria containing the pGLO plasmid by growing them on +arabinose plates. pGLO is made up of three genes that are joined together using recombinant DNA technology. They are as follows: -Bla, which codes for the ...
http://en.wikipedia.org/wiki/PGLO
- Bacterial Transformation Lab
The pGLO Plasmid. The plasmid used in this exercise has been modified by removing the structural genes of the arabinose operon (ara A, ara B, and ara D). These genes were replaced by a gene from a bioluminescent jellyfish that codes for ...
http://faculty.clintoncc.suny.edu/faculty/michael.gregory/files/bio%20101/bio%..
- Why use arabinose in the plate in pglo experiments
Why use arabinose in the plate in pglo experiments? In: Biology, Microbiology [ Edit categories]. Answer: Improve. The arabinose sugar binds to the RNA in the cell and helps turn on the gene expression to make GFP, green flourescent ...
http://wiki.answers.com/Q/Why_use_arabinose_in_the_plate_in_pglo_experiments
- Protocol for pGLO Transformation
The pGLO plasmid also carries the gene araC, which produces a protein needed for transcription of genes in the presence of arabinose sugar. The purpose of this protein is discussed below. Transcriptional Gene Regulation: In many cases, ...
http://www.westminster.edu/acad/sim/pdf/SpGLOTransformation.pdf
- Bacterial Transformation Lab
pGLO also includes a positive gene regulation system, which can be used to control expression of the green fluorescent protein in transformed cells. In this system, the sugar arabinose turns the GFP gene on. The GFP gene is cloned behind ...
http://www.tmcc.edu/biology/downloads/documents/bio100/BIOL100BacterialTransLa..
- Transformation of pGlo into Escherichia coli
pGlo also incorporates a special gene regulation system which can be used to control expression of the fluorescent protein in transformed cells. The gene can easily be switched on in transformed cells by adding the sugar arabinose to the ...
http://www.microeco.uzh.ch/uni/kurs/bio3_05/berichte/11-Di-D-report05.pdf
- pglo.htm
This unique ability is based on the pGLO plasmid, which carries a gene for the production of Green Fluorescent Protein (GFP). The pGLO system incorporates an arabinose promoter which precisely controls expression of the GFP in ...
http://faculty.sdmiramar.edu/dtrubovitz/micro/pglo/
- pGLO Bacterial Transformation Kit - Bio-Rad Laboratories
The genes are activated only when arabinose is present in the environment. Bio- Rad's pGLO plasmid incorporates the arabinose promoter, but the genes involved in the breakdown of arabinose have been replaced with the jellyfish gene ...
http://www3.bio-rad.com/B2B/BioRad/product/br_category.jsp?BV_SessionID=@@@@10..
- Genetic transformation of Escherichia coli with pGLO
Figure 1: A map of the pGLO plasmid. Abbreviations: ori, origin of replication; bla, beta-lactamase gene; gfp, green fluorescent protein gene; AraC, promoter sequence of the E. coli ara operon. In the presence of the sugar arabinose, the ...
http://iiumedic.com/web/v1/wp-content/uploads/2009/08/5-bactransformation.pdf
- Bacterial Transformation - IndiaStudyChannel
Cells that inherit the pGLO take on fluorescence and ampicillin resistance. There is a molecular switch on the plasmid, and it is activated by arabinose sugar. When the transformed cells are grown in a medium with arabinose the pGLO genes ...
http://www.indiastudychannel.com/projects/264-Bacterial-Transformation.aspx
